LSP248 · Microscope and optical tweezers research instruments · Microscopic imaging and particle research
Reusable modules

Comparison of morphology of probe exit light spots

Facing the microfiber probe, taking the spot shape and relative distribution as the observation route, the near field center and main axis width are planned to be output. The page is for project evaluation and does not mean that the development has been completed or has been accepted on site.

Project Judgment Summary

Product/DeviceMicrofiber probe
Measure routeBM · Spot shape and relative distribution
Ability GradingReusable modules
Verification statusThe actual machine/product domain acceptance of this scenario has not been carried out
Scene nature:Similar potential application scenarios for laser spots. The potential application scenario has not yet been developed into a complete product system; the actual machine/product domain acceptance of this scenario has not been carried out. The actual product/product domain acceptance quantity in catalog statistics is 0.

Business tasks and intended output

target audience
Microfiber probe: spot shape and relative distribution
pseudo output
Near field center and main axis width
Applicable conditions
Biological tissue components cannot be determined from light spots
priority
General extension. Verification needs to be completed according to the listed optical conditions, calibration and samples.

Imaging input and algorithm routing

Imaging and device conditions

Linear response imaging, fixed attenuation and background acquisition avoid saturation and ROI truncation. Microscopes, relay sampling and wavelength-adapted cameras

BM · Spot shape and relative distribution

Linear response imaging, fixed attenuation and background acquisition to avoid saturation and ROI truncation

  • A101Adaptive threshold laser spot grayscale centroid
  • A105Laser spot second moment and main axis diameter
  • A106Laser spot surrounding energy diameter
  • A107Laser spot quality and usability diagnostics

Existing algorithmic evidence:A101–A112 only complete analytical synthesis spot and controlled geometry verification; see laser_spot_benchmark.json

Current capabilities and capabilities to be supplemented

Reusable modules

Existing modules can be used as hosts

Existing light point positioning, quality diagnosis or statistical modules can be used as the main calculation basis; ROI, calibration, threshold and interface verification still need to be completed by device.

Gaps in this scenario:Pixel scale, background subtraction and dynamic range verification required; grayscale integration is not watts or joules. The components of biological tissue cannot be determined from the light spot. Particle image positioning is an algorithm migration and the image model must be re-verified.

Route-level gaps:Pixel scale, background subtraction and dynamic range verification required; grayscale integration not watts or joules

"Reusable modules" only describe the relative development volume and do not constitute a commitment to accuracy, tempo, stability or delivery time.

Verification methods and acceptance prerequisites

Verify center, width and cutoff separately against a stand-alone beam analyzer or known spot pattern. Microsphere stage, microscopic ruler and independent positioning reference

Acceptance itemsEvidence required for project confirmationCurrent status
Inputs and samplesReal device original frames, normal/abnormal samples, exposure and light path conditions, sample grouping and set asideNot provided, waiting for project input
Magnitude and ErrorIndependent reference value, calibration record, error statistical caliber and mutual confirmation thresholdNot verified
Exceptions and boundariesAnticipated handling of missed detections, false detections, overexposure, occlusion, reflection, out-of-view and communication abnormalitiesTo be defined
System integrationInterface, time synchronization, deployment platform, tempo, log, version and continuous running recordNot integrated

Evidence sources and citation boundaries

The source supports measurement principles or similar applications; the module combination, device and acceptance method in this scenario are engineering derivation, and product verification has not been completed.

LS-S20 · Original factory/scientific research institution/original paper

Radial Symmetry Center Original Paper

Sub-pixel center positioning of approximately radially symmetric targets

Verification date: 2026-10-24

View original public information →
LS-S21 · Original factory/scientific research institution/original paper

Research on position calibration of optical tweezers camera

Limited particle position detection and the impact of exposure and sampling on calibration

Verification date: 2026-10-24

View original public information →
LS-S30 · Original factory/scientific research institution/original paper

Ophir small beam measurement

Optical conditions in small beam near-field, far-field and mode field measurements

Verification date: 2026-10-24

View original public information →
Source usage scope:The public information is used to support the measurement principle or the existence of similar applications, and does not prove that this scenario has been developed, deployed or accepted by Jishi Technology.
Project consultation

Define the Development Scope with Samples and Device Requirements

Comparison of morphology of probe exit light spots

Please describe the task object, original picture or video, target indicators, device platform, interface and sample situation.

Phone: 13910119357
xuzhiyang0928@gmail.com

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