Radial Symmetry Center Original Paper
Sub-pixel center positioning of approximately radially symmetric targets
Verification date: 2026-10-24
View original public information →LSP241 · Microscope and optical tweezers research instruments · Microscopic imaging and particle research
Reusable modules
Alignment of excitation beam with pinhole fiducial
Facing the confocal light path, taking the target surface light point position as the observation route, the conjugate surface light point offset is intended to be output. The page is for project evaluation and does not mean that the development has been completed or has been accepted on site.
Project Judgment Summary
Product/DeviceConfocal light path
Measure routeXY · Target surface light spot position
Ability GradingReusable modules
Verification statusThe actual machine/product domain acceptance of this scenario has not been carried out
Scene nature:Similar potential application scenarios for laser spots. The potential application scenario has not yet been developed into a complete product system; the actual machine/product domain acceptance of this scenario has not been carried out. The actual product/product domain acceptance quantity in catalog statistics is 0.
Business tasks and intended output
- target audience
- Confocal light path: target spot position
- pseudo output
- Conjugate surface light point offset
- Applicable conditions
- Actual axial resolution requires separate measurement of fluorescent samples
- priority
- General extension. Verification needs to be completed according to the listed optical conditions, calibration and samples.
Imaging input and algorithm routing
Imaging and device conditions
Fix the camera and target surface, record the exposure and gain, and cover the light spot and background in the field of view. Microscopes, relay sampling and wavelength-adapted cameras
XY · Target surface light spot position
Fix the camera and target surface, record exposure and gain, and cover the light spot and background in the field of view
- A101Adaptive threshold laser spot grayscale centroid
- A107Laser spot quality and usability diagnostics
- A112Laser spot two-dimensional repeatability and coverage ellipse
Existing algorithmic evidence:A101–A112 only complete analytical synthesis spot and controlled geometry verification; see laser_spot_benchmark.json
Current capabilities and capabilities to be supplemented
Reusable modules
Existing modules can be used as hosts
Existing light point positioning, quality diagnosis or statistical modules can be used as the main calculation basis; ROI, calibration, threshold and interface verification still need to be completed by device.
Gaps in this scenario:ROI, ruler/plane calibration and reference point definition are required; only the two-dimensional position of the target surface can be directly observed. The actual axial resolution needs to be measured separately on fluorescent samples. Particle image positioning is an algorithm migration and the image model must be re-verified.
Route-level gaps:Requires ROI, ruler/plane calibration and reference point definition; can only directly observe the two-dimensional position of the target surface
"Reusable modules" only describe the relative development volume and do not constitute a commitment to accuracy, tempo, stability or delivery time.
Verification methods and acceptance prerequisites
Test independently with a two-dimensional displacement stage and a fixed reference point, reporting center error P95, missed detections and false detections. Microsphere stage, microscopic ruler and independent positioning reference
| Acceptance items | Evidence required for project confirmation | Current status |
|---|---|---|
| Inputs and samples | Real device original frames, normal/abnormal samples, exposure and light path conditions, sample grouping and set aside | Not provided, waiting for project input |
| Magnitude and Error | Independent reference value, calibration record, error statistical caliber and mutual confirmation threshold | Not verified |
| Exceptions and boundaries | Anticipated handling of missed detections, false detections, overexposure, occlusion, reflection, out-of-view and communication abnormalities | To be defined |
| System integration | Interface, time synchronization, deployment platform, tempo, log, version and continuous running record | Not integrated |
Evidence sources and citation boundaries
The source supports measurement principles or similar applications; the module combination, device and acceptance method in this scenario are engineering derivation, and product verification has not been completed.
Research on position calibration of optical tweezers camera
Limited particle position detection and the impact of exposure and sampling on calibration
Verification date: 2026-10-24
View original public information →Ophir small beam measurement
Optical conditions in small beam near-field, far-field and mode field measurements
Verification date: 2026-10-24
View original public information →Source usage scope:The public information is used to support the measurement principle or the existence of similar applications, and does not prove that this scenario has been developed, deployed or accepted by Jishi Technology.
Project consultation
xuzhiyang0928@gmail.com
Define the Development Scope with Samples and Device Requirements
Alignment of excitation beam with pinhole fiducial
Please describe the task object, original picture or video, target indicators, device platform, interface and sample situation.
Phone: 13910119357xuzhiyang0928@gmail.com