Radial Symmetry Center Original Paper
Sub-pixel center positioning of approximately radially symmetric targets
Verification date: 2026-10-24
View original public information →LSP244 · Microscope and optical tweezers research instruments · Microscopic imaging and particle research
Need to add new coreAlgorithm migration · Non-laser product applications
Capturing the 2D position timing front end of microspheres
Facing the optical tweezers experimental device, using point-like particle imaging migration as the observation route, it is planned to output microsphere trajectories and missing frames. The page is for project evaluation and does not mean that the development has been completed or has been accepted on site.
Project Judgment Summary
Product/DeviceOptical tweezers experimental device
Measure routePARTICLE · Point-like particle imaging migration
Ability GradingNeed to add new core
Verification statusThe actual machine/product domain acceptance of this scenario has not been carried out
Migration instructions:The positioning algorithm migration of particle/luminous point images should not be expressed as a proven laser product application. The potential application scenario has not yet been developed into a complete product system; the actual machine/product domain acceptance of this scenario has not been carried out. The actual product/product domain acceptance quantity in catalog statistics is 0.
Business tasks and intended output
- target audience
- Optical tweezers experimental device: point-like particle imaging migration
- pseudo output
- Microsphere trajectories and missing frames
- Applicable conditions
- The trap stiffness requires exposure compensation and dynamic calibration.
- priority
- Special development. The measurement/control core needs to be supplemented and verified according to the special project approval
Imaging input and algorithm routing
Imaging and device conditions
Particle images can be separated under laser illumination, and microscopic ruler, exposure and sampling calibration can be performed. Microscopes, relay sampling and wavelength-adapted cameras
PARTICLE · Point-like particle imaging migration
Particle images can be separated under laser illumination, microscopic ruler, exposure and sampling calibration
- A22Kalman filter
- A25Welch power spectrum estimation
- A102Multi-laser spot connected domain candidate extraction
- A104Gradient radial symmetry light spot center
- A108One-to-one matching of multi-laser spot reference bits
Existing algorithmic evidence:A101–A112 only complete analytical synthesis spot and controlled geometry verification; see laser_spot_benchmark.json
Current capabilities and capabilities to be supplemented
Need to add new core
Need to add core computing
Light point extraction can only be used as an observation front-end, and core capabilities such as three-dimensional reconstruction, closed-loop control, wavefront or physical quantity inversion need to be developed and verified separately.
Gaps in this scenario:Particle correlation/dynamics/blur compensation needs to be added; the particle image is migrated by the positioning algorithm and cannot be used as the center of the original laser beam. The trap stiffness requires exposure compensation and dynamic calibration. Particle image positioning is an algorithm migration and the image model must be re-verified.
Route-level gaps:Particle correlation/dynamics/blur compensation needs to be added; the particle image is migrated by the positioning algorithm and cannot be used as the center of the original laser beam
"New cores required" only describes the relative development volume and does not constitute a commitment to accuracy, tempo, stability or delivery time.
Verification methods and acceptance prerequisites
Microspheres are driven by a displacement stage and tested with independent trajectory marking, and reference fluid/force calibration is used for physical property inversion. Microsphere stage, microscopic ruler and independent positioning reference
| Acceptance items | Evidence required for project confirmation | Current status |
|---|---|---|
| Inputs and samples | Real device original frames, normal/abnormal samples, exposure and light path conditions, sample grouping and set aside | Not provided, waiting for project input |
| Magnitude and Error | Independent reference value, calibration record, error statistical caliber and mutual confirmation threshold | Not verified |
| Exceptions and boundaries | Anticipated handling of missed detections, false detections, overexposure, occlusion, reflection, out-of-view and communication abnormalities | To be defined |
| System integration | Interface, time synchronization, deployment platform, tempo, log, version and continuous running record | Not integrated |
Evidence sources and citation boundaries
The source supports measurement principles or similar applications; the module combination, device and acceptance method in this scenario are engineering derivation, and product verification has not been completed.
Research on position calibration of optical tweezers camera
Limited particle position detection and the impact of exposure and sampling on calibration
Verification date: 2026-10-24
View original public information →Ophir small beam measurement
Optical conditions in small beam near-field, far-field and mode field measurements
Verification date: 2026-10-24
View original public information →Source usage scope:The public information is used to support the measurement principle or the existence of similar applications, and does not prove that this scenario has been developed, deployed or accepted by Jishi Technology.
Project consultation
xuzhiyang0928@gmail.com
Define the Development Scope with Samples and Device Requirements
Capturing the 2D position timing front end of microspheres
Please describe the task object, original picture or video, target indicators, device platform, interface and sample situation.
Phone: 13910119357xuzhiyang0928@gmail.com