Radial Symmetry Center Original Paper
Sub-pixel center positioning of approximately radially symmetric targets
Verification date: 2026-10-24
View original public information →LSP243 · Microscope and optical tweezers research instruments · Microscopic imaging and particle research
Partially reusable
Two-dimensional registration of microscopic field of view and laser spot
Facing the microlaser processing platform, the two-dimensional command/target surface coordinate calibration is used as the observation route, and the residual error of the microscopic XY coordinate is planned to be output. The page is for project evaluation and does not mean that the development has been completed or has been accepted on site.
Project Judgment Summary
Product/DeviceMicrolaser processing table
Measure routeCAL · Two-dimensional command/target coordinate calibration
Ability GradingPartially reusable
Verification statusThe actual machine/product domain acceptance of this scenario has not been carried out
Scene nature:Similar potential application scenarios for laser spots. The potential application scenario has not yet been developed into a complete product system; the actual machine/product domain acceptance of this scenario has not been carried out. The actual product/product domain acceptance quantity in catalog statistics is 0.
Business tasks and intended output
- target audience
- Microlaser processing table: two-dimensional command/target surface coordinate calibration
- pseudo output
- Microscopic XY coordinate residuals
- Applicable conditions
- The objective lens magnification and cover glass need to be fixed
- priority
- General extension. Verification needs to be completed according to the listed optical conditions, calibration and samples.
Imaging input and algorithm routing
Imaging and device conditions
Cover the calibrated corresponding points and independent set-out points of the two-dimensional field of view, and fix the optical path and working plane. Microscopes, relay sampling and wavelength-adapted cameras
CAL · Two-dimensional command/target coordinate calibration
Calibration corresponding points and independent set-out points covering the two-dimensional field of view, fixed optical path and working plane
- A101Adaptive threshold laser spot grayscale centroid
- A107Laser spot quality and usability diagnostics
- A109Two-dimensional pixel to physical coordinate polynomial calibration
- A110laser spot calibration mapping and covariance propagation
- A112Laser spot two-dimensional repeatability and coverage ellipse
Existing algorithmic evidence:A101–A112 only complete analytical synthesis spot and controlled geometry verification; see laser_spot_benchmark.json
Current capabilities and capabilities to be supplemented
Partially reusable
Requires calibration, synchronization or integration
The light point detection front-end or timing statistics module can be reused, but the project still needs to add geometric calibration, time synchronization, motion compensation or equipment system integration.
Gaps in this scenario:Calibration acquisition, coordinate definition and verification are required; two-dimensional empirical mapping does not represent any three-dimensional or robot calibration. The objective lens magnification and cover glass need to be fixed. Particle image positioning is an algorithm migration and the image model must be re-verified.
Route-level gaps:Calibration acquisition, coordinate definition and verification are required; two-dimensional empirical mapping does not represent any three-dimensional or robot calibration
"Partially reusable" only describes the relative development volume and does not constitute a commitment to accuracy, tempo, stability or delivery time.
Verification methods and acceptance prerequisites
Separate calibration points and set-out points to check edge, back-and-forth, temperature and repeated clamping errors. Microsphere stage, microscopic ruler and independent positioning reference
| Acceptance items | Evidence required for project confirmation | Current status |
|---|---|---|
| Inputs and samples | Real device original frames, normal/abnormal samples, exposure and light path conditions, sample grouping and set aside | Not provided, waiting for project input |
| Magnitude and Error | Independent reference value, calibration record, error statistical caliber and mutual confirmation threshold | Not verified |
| Exceptions and boundaries | Anticipated handling of missed detections, false detections, overexposure, occlusion, reflection, out-of-view and communication abnormalities | To be defined |
| System integration | Interface, time synchronization, deployment platform, tempo, log, version and continuous running record | Not integrated |
Evidence sources and citation boundaries
The source supports measurement principles or similar applications; the module combination, device and acceptance method in this scenario are engineering derivation, and product verification has not been completed.
Research on position calibration of optical tweezers camera
Limited particle position detection and the impact of exposure and sampling on calibration
Verification date: 2026-10-24
View original public information →Ophir small beam measurement
Optical conditions in small beam near-field, far-field and mode field measurements
Verification date: 2026-10-24
View original public information →Source usage scope:The public information is used to support the measurement principle or the existence of similar applications, and does not prove that this scenario has been developed, deployed or accepted by Jishi Technology.
Project consultation
xuzhiyang0928@gmail.com
Define the Development Scope with Samples and Device Requirements
Two-dimensional registration of microscopic field of view and laser spot
Please describe the task object, original picture or video, target indicators, device platform, interface and sample situation.
Phone: 13910119357xuzhiyang0928@gmail.com